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Isolation, Screening and Morphological characterization of Laccase producing fungi

    Authors

    • Bhuneshwari Nayak 1
    • Rachana Choudhary 1
    • Roymon M. G. 2

    1 Department of Microbiology, Shri Shankaracharya Mahavidyalaya, Junwani, Bhilai, Chhattisgarh, India

    2 Principal, St. Thomas College, Ruabandha, Bhilai, Chhattisgarh, India

,

Document Type : Research Article

10.47392/irjash.2022.008
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Abstract

Research was carried out on the production of extracellular laccase enzyme by fungi isolated from different lignocellulolytic waste soils within the Bhilai- Durg region of Chhattisgarh, India. Soil samples were collected from lignocel- lulolytic waste such as flower, citrus peel and paddy straw soil and immediately transported to laboratory for analysis. Fungal colony was isolated by the serial dilution method on potato dextrose agar. A total of 49 fungal cultures were isolated from the soil samples. Isolated fungal culture were screened for pro- duction of laccase enzyme in guaiacol containing potato dextrose agar plate. Laccase producing fungi was morphological identified by Agharkar Research Institute in Pune. The fungal isolates were identified as Scytalidium lignicola, Humicola fuscoatra, Fusarium sp., Paecilomyces sp., Curvularia sp., Fusar- ium sp., Aspergillus niger, Chaetomium sp., Penicillium sp. And Histoplasma capsulatum

Keywords

  • Extracellular
  • lignocellulolytic
  • laccase
  • Production
  • morphology

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References
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Andlar, Martina, et al. “Lignocellulose degrada- tion: An overview of fungi and fungal enzymes involved in lignocellulose degradation”. Engi- neering in Life Sciences 18.11 (2018): 768–778. 10.1002/elsc.201800039.
Ayla, Sridevi, Narasimha Golla, and Suvar- nalathadevi Pallipati. “Production of Ligninolytic Enzymes from Penicillium Sp. and Its Effi- ciency to Decolourise Textile Dyes”. The Open Biotechnology Journal 12.1 (2018): 112–122. 10.2174/1874070701812010112.
Bello, A., et al. “Screening and Identification of Lac- case Producing Fungi from Environmental Sam- ples”. UMYU Journal of Microbiology Research (UJMR) 6.1 (2021): 91–98. 10.47430/ujmr.2161.012.
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International Research Journal on Advanced Science Hub
Volume 4, Issue 02
February 2022
Page 38-43
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History
  • Receive Date: 22 January 2022
  • Revise Date: 17 February 2022
  • Accept Date: 20 February 2022
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  • Article View: 548
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APA

Nayak, B. , Choudhary, R. and M. G., R. (2022). Isolation, Screening and Morphological characterization of Laccase producing fungi. International Research Journal on Advanced Science Hub, 4(02), 38-43. doi: 10.47392/irjash.2022.008

MLA

Nayak, B. , , Choudhary, R. , and M. G., R. . "Isolation, Screening and Morphological characterization of Laccase producing fungi", International Research Journal on Advanced Science Hub, 4, 02, 2022, 38-43. doi: 10.47392/irjash.2022.008

HARVARD

Nayak, B., Choudhary, R., M. G., R. (2022). 'Isolation, Screening and Morphological characterization of Laccase producing fungi', International Research Journal on Advanced Science Hub, 4(02), pp. 38-43. doi: 10.47392/irjash.2022.008

CHICAGO

B. Nayak , R. Choudhary and R. M. G., "Isolation, Screening and Morphological characterization of Laccase producing fungi," International Research Journal on Advanced Science Hub, 4 02 (2022): 38-43, doi: 10.47392/irjash.2022.008

VANCOUVER

Nayak, B., Choudhary, R., M. G., R. Isolation, Screening and Morphological characterization of Laccase producing fungi. International Research Journal on Advanced Science Hub, 2022; 4(02): 38-43. doi: 10.47392/irjash.2022.008

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